Dear Netters,
I'm working with an uncharacterized yeast enzyme activity, and it seems
to have activity around pH 2.5 and 3.0. Currently I'm using Citric Acid
and Citrate-Phosphate buffers to get a decent result. The activity is,
however, inconsistent-standard deviation is high from experiment to exp.
I guess that harsh pH like 2.5 itself might have killed the enzyme
activity to give varying results, but is that possible? If pH itself
were the matter here, it should always kill the enzyme, and if there is
the enzyme activity around this pH, the enzyme should survive at this pH.
So I suspect the buffers (citrate and citrate-phosphate) may not be good
at this pH. Is there anyone who had this kind of experience, and/or who
could recommend a good buffer for this pH range (2.5-3.0). Thank you in
advance for comments.
PS: Please send an email to me in addition to posting to the newsgroups
if you will do so.
************************************************************************
* Namjin Chung * *
* Program in Molecular Cancer Biology * Email: nc1 at acpub.duke.edu *
* Duke University Medical Center, Box 3345 * Voice: +1 (919) 684-2363 *
* Durham, NC 27710 * Fax : +1 (919) 681-8253 *
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