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beta gal assay

Ray Lu luray at duke.usask.ca
Tue Jul 9 13:23:04 EST 1996


Recently, I have trouble reproducing my beta gal results in my
yeast two hybrid system (from Clontech). I use ONPG as substrate.
I have tried a few times now. All samples turned out to be negative,
even for the ones that have previously shown positive.

I have remade all solutions fresh and retransformed yeast, but it 
didn't help. Where can go wrong? Anyone has similar experience?

------
Ray LU
University of Saskatchewan
Dept of Vet Micro, WCVM
Sasktoon, SK S7N 5B4 Canada
Tel (306)966-7228
Fax (306)966-7244
luray at duke.usask.ca





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