Recently, I have trouble reproducing my beta gal results in my
yeast two hybrid system (from Clontech). I use ONPG as substrate.
I have tried a few times now. All samples turned out to be negative,
even for the ones that have previously shown positive.
I have remade all solutions fresh and retransformed yeast, but it
didn't help. Where can go wrong? Anyone has similar experience?
University of Saskatchewan
Dept of Vet Micro, WCVM
Sasktoon, SK S7N 5B4 Canada
luray at duke.usask.ca