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Antibodies

David Botstein botstein at genome.stanford.edu
Sun Jul 7 14:50:20 EST 1996


My lab has had the impression that rabbits 
often have been exposed/infected with yeasts
as judged from the antibodies present in serum
controls taken before immunization. I doubt seriously
whether this depends on the sex of the rabbit, as
both males and females have been used in this way.

Secondly, yeast researchers should be aware that the
"yeast" that infect female urinary tracts in humans
are only very distantly related to Saccharomyces or
Schizosaccharomyces (the usual experimental organisms).

Finally, when the pre-immune antibodies are likely to
be a serious problem, one might consider using guinea
pigs in place of rabbits to raise the antibodies. We
have had good fortune with this species both at getting
useful antibodies and low background. This is especially
a valuable trick for glycoproteins that reside on the
surface of yeast.

All that said, everyone should realize that very many
yeast researchers have produced excellent antibodies
with acceptable pre-immune titers without getting
exotic about sexing the rabbits or using other species.

Good luck

David Botstein



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