=09Try the procedure proposed by STUCKA and FELDMAN, "Preparation of=20
yeast high molecular weight DNA" wich will alow you to prepare DNA from=20
150 to 200 kb.
=09The protocol is from:
JOHNSTON, J.R. Molecular Genetics of Yeast (The Practical Aproach=20
series), Chapter 3 "Cosmid Cloning of yeast DNA". IRL Press, 1994
=09I've used it with very good results...
=09Hope it can help you.
=09\ Juan Lucas Argueso jlagalme at carpa.ciagri.usp.br /=20
/ Departamento de Gen=E9tica \
\ Escola Superior de Agricultura "Luiz de Queiroz" /
/ Universidade de S=E3o Paulo, Piracicaba - SP, Brasil \