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isolation of large DNA

Juan Lucas Argueso jlagalme at ciagri.usp.br
Mon Jul 1 10:50:41 EST 1996

=09Try the procedure proposed by STUCKA and FELDMAN, "Preparation of=20
yeast high molecular weight DNA" wich will alow you to prepare DNA from=20
150 to 200 kb.
=09The protocol is from:

JOHNSTON, J.R.  Molecular Genetics of Yeast (The Practical Aproach=20
series), Chapter 3 "Cosmid Cloning of yeast DNA". IRL Press, 1994
=09I've used it with very good results...
=09Hope it can help you.

=09\   Juan Lucas Argueso  jlagalme at carpa.ciagri.usp.br   /=20
        /              Departamento de Gen=E9tica                \
        \   Escola Superior de Agricultura "Luiz de Queiroz"   /
        /  Universidade de S=E3o Paulo, Piracicaba - SP, Brasil  \

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