We make a stock of 3-AT (1M) in water and add it to plates just before
pouring. However, 3-AT goes "bad" quickly (in a week), so if we aren't
going to use all the plates right away we just spread some 3-AT onto the
plates before using. Hope this helps.
University of Utah
Dept. of Onc. Sci.
helen.mcbride at genetics.utah.edu
"Where the telescope ends, the microscope begins, which of the two has
the grander view?" Victor Hugo