IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Liquid B-gal assays???? How to?

mike at ncsu.edu mike at ncsu.edu
Thu Dec 12 12:11:33 EST 1996

I would appreciate your sharing your favorite protocol for liquid
B-gal assays with me.  I am using PJ69.4a for a 2-hybrid screen and
will potentially being doing many at one time (or hopefully a few).  I
have tried grinding the cells with glass beads and then incubating the
extract with X-gal and Z buffer but it didn't work out (have yet to
repeat this).  Just wondering if anyone knows of a good way to do

Mike Faughn

Dept. Genetics, NCSU

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net