Hi Peter
Two alternatives exist: Lyticase (BoehringerM) is a cheaper form
of zymolyase, while glusulase (Sigma?) is also used in spheroplasting. I have
used both, to spheroplast yeast before lysing with phenol/acid-washed glass
beads, but not for the purpose of transfection.
Good luck.
CAS
PS. If you don't have much joy with spheroplasting for trasnfection, I have a
guaranteed LiAc protocol, well-optimised, which we routinely use for library
screens - as much as 10^7 transformants from 75ug DNA.
