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Help Me ! / Yeast transformation for Two-Hybrid screening

Vladimir Svetlov vvsvetlov at utmem1.utmem.edu
Wed Aug 21 20:36:42 EST 1996


In article <naganot-1908962026120001 at molnb03.bri.niigata-u.ac.jp>,
naganot at bri.niigata-u.ac.jp (Takashi Nagano) wrote:


:> SOMEONE WHO KNOWS an optimal or better protocol and/or SD medium recipe
:> for transforming CG1945 yeast to get higher efficiency, PLEASE TEACH ME
:> HOW THEY ARE!
> 
:> Thank you very much for your help ! 
:> My e-mail address is : naganot at bri.niigata-u.ac.jp
> 
:> Takashi Nagano,
:> Niigata, JAPAN

A protocol claiming up to 22 000 000 transformants per mcg of plasmid DNA
is offered by R.D.Gietz lab homepage. 

Regards,
VS



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