Hi there. I want to screen an inducible Arabidopsis cDNA library in yeast.
Looking through the literature, the promoter (GAL4) is induced in liquid
culture lacking glucose with 1% galactose. Does anybody have any experience
in reducing this galactose concentration. How low can I go before the
promoter is turned off? and what conc of glucose will turn off
the promoter?? Thanks.
