Hi folks,
Thanks for all that replied my message about geneticin. It really
worked out. One of the strains was sensitive to 50ug/ml and the other to
100ug/ml in liquid YPD medium. I think it's ok and I intend to analyze
them in solid medium before the transformation.
Now I have another question: a colleague of mine wants to
do a plasmid extraction directly from yeast without the step that
involves bacteria transformation. Does anybody know about any method? She
can't select the gene she is cloning in bacteria because it is letal for
this organism. If somebody could help us, we'd appreciate a lot.
Thanks in advance,
Solange
