Hi.
I am currently in the process of setting up the Two Hybrid System. I expressed
my BD-protein of interest in strain CG1954 and want to do PCR directly from
colonies. The question I have is: how can I lyse the cells so that I can do
PCR directly (without having to do plasmid isolation first and then PCR)? High
temp or low temp? Neither?
Any advice you could give would be greatly appreciated.
Thanks
Kelley and Iveta
(please email responses to iiontche at acs.bu.edu)
