In article
<Pine.A32.3.91.960730234254.150268A-100000 at login1.email.unc.edu>, Scott
Kaufmann <shkaufma at email.unc.edu> wrote:
> Hi all. I am trying to create yeast expression vectors using promoters
> (both ADH and GAL1) from various yeast two-hybrid vectors and some RS
> shuttle vectors that we have in our lab. I have noticed that these
> vectors use various fragments of the promoter and was wondering if
> anyone had some advice as to the best length of each promoter for the
> highest expression.
>> By the way, if someone already has some expression vectors that they are
> willing to share, it would be greatly appreciated.
>> Thanks,
>> Scott Kaufmann
> Universtiy of North Carolina
> at Chapel Hill
Hi Scott!
I'm a student at the Gietz lab (University of Manitoba) and we may be able
to give you a hand. We have a number of ADH and GAL1 expression vectors
that may be of use to you. They are made from the YXPlac vectors made by
Dr. Gietz and work well! You can take a look at the YXPlac vectors at the
web site:
http://www.umanitoba.ca/faculties/medicine/human_genetics/gietz/Trafo.html
(It also has some nifty high-efficiency trafo protocols if you're interested.)
Could you tell us a little bit more about what you are trying to express
to know which vectors would be the best to send? (An address to send them
to would also be helpful.)
Good luck!
Melanie Richard (mrichar at mail.cc.umanitoba.ca.)
Department of Human Genetics
University of Manitoba
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