Dear Drgon:
Working with yeast strain W303 I noticed that there is always
considerable background growth when cells are plated on minimal FOA
medium plus uracil. If you plate too few cells it might be difficult to
pick a good crop of FOA resistant (ura3-) cells but they will come out
very clear. If more cells are plated you must wait up to 1 week to pick
the colonies that rise above the background, repurify them and re test on
minimal - uracil. This residual growth might be starin dependent.
F.G. Nobrega
Universidade de Sao Paulo
Departamento de Microbiologia - ICB II / USP
Av. Prof. Lineu Prestes, 1374
05508-900 Sao Paulo - SP - Brasil
Voice: (011) 818-7588 Fax: 55-11-818-7354 E-mail: fgdnobre at usp.br
On Thu, 1 Aug 1996, Tomas Drgon wrote:
> Hi folks.
> I have a question: How many cells can you plate on FOA plate and still get
> selection? Is there an upper limit?
>> Tomas Drgon
>> --
> Vinarske zavody Pezinok pozdravuju vsetky pokrokove sily sveta!
>>
