G'day to all,
Does anybody have "the ultimate" method to obtain high efficiency competent
CG1945 yeasts for the 2 hybrid system. I am doing a sequential
transformation rather than co-transformation. I cannot, so far, obtain a
transformation efficiencies of > 10 to the 3 or 4 and, as a result, I am
forced to do many transformations. I am following the method descibed in the
Matchmaker protocol pamphlet.