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PCR of inserts in pACTII

Nico Stuurman stuurman at ubaclu.unibas.ch
Sun Oct 29 16:21:17 EST 1995


Did anyone succesfully amplify inserts in the 2-hybrid vector pACTII? 
If so, could you please share the primer sequences and PCR conditions
that you used?  Is it possible to use transformed yeast colonies as a 
template?  Many thanks for your help.

Nico Stuurman
M.E. Mueller Institut at the Biozentrum Basel, Basel, CH
stuurman at ubaclu.unibas.ch



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