Hello. I am a graduate student beginning in a study of subunit
interactions among an oligomeric protein via the two-hybrid system. We
have purchased the Clonetech Matchmaker I system and I have already had
some success with cloning my subunit cDNAs, yet..... All I read here and
elsewhere indicates that these are not the best vectors, especially for
confirming by Western blot. Also, most of the publications I read about
protein interactions determined by two-hybrid seem to use Brent's LexA
interaction trap system. Is this a result of my incomplete literature
search or is it really the case. If it is true what are the advantages of
LexA over Gal4??
Any comments would be appreciated!!
e-mail: NAYERS at UTMEM1.UTMEM.EDU