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Need recipe for low pH YPD plates

Luiz H. Gomes luhgomes at CARPA.CIAGRI.USP.BR
Mon Oct 23 15:20:47 EST 1995


	Hi, You must do a YPD recipe as usual but for each 100 ml, you 
complete to 80 ml of water, esterilizes and completes to 100ml with 
the addition of phosphate-citrate buffer, esterilized separated, 
made as following:

Phosphate-citrate Buffer
3.9 % citric acid
0.22 % Na2HPO4
 Your final pH will be 2,5 and it will be solid.

	If you need anything else, ask,
	Good Luck!

Luiz Humberto Gomes
Yeast Laboratory
Genetics Deps.
ESALQ/USP




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