Dear Kent et al.,
We have been amplifying products up to 2 kb from S. pombe using a small
portion of a freshly grown colony in a standard PCR reaction. While we often
start with Mg++ curve, our typical result is that no additional Mg++ is
required beyond that in the 10X buffer. We use VENT, so I don't know if Taq
or Pfu users have a different experience. Good luck.
"I'd rather be fission."
Does anyone have info regarding colony PCR with S. cerevisiae when
expected products are 1000 bp or more?