In article <gbutler-181095091433 at gbutlermac.ucd.ie>,
gbutler at macollamh.ucd.ie (Geraldine Butler) wrote:
> In article <tomasd-0609560945320001 at fp5-9n.domain>,
>tomasd at bdg10.niddk.nih.gov (Tomas Drgon) wrote:
> > I use mouse antiHa from Boehringer (cat#1583 816), 1:10000 for 30 min at
> > RT, 2-nd antibody goat anti-mouse coupled w HRP, nickel enhanced
> > Tomas Drgon
> > --
> > Signature under construction...
> I'm interested in the reference to the Boehringer antiHA antibody, at a
> dilution of 1:10000. The instructions with this antibody say to use 5ug/ml
> - as they supply 200ug in a standard pack, this doesn't allow many
> westerns! Have many people tried it at dilutions such as 1:10000?
> Geraldine Butler University College Dublin Ireland
Sorry, sorry, my fault. I mixed up things. I also use the Boehringer
antiHa at 5 ug/ml, overnight in the coldroom.
Thanks for correction, and sorry again.
Signature under construction...