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YPG with high CaCl2 conc.

PAIDH001 at MC.DUKE.EDU PAIDH001 at MC.DUKE.EDU
Sat Oct 14 11:51:02 EST 1995


John,
I have isolated some strains which are sensitive to CaCl2 (100 and 200mM) as 
suppressors of cdc1-1(ts). To prevent the precipitation of CaCl2 we use YPD 
plates pH5.5 using 50mM Succinate/NaOH buffer (Calcium precipitates as its 
phosphate salt and this is soluble at lower pH). This is described in Cunningham 
and Fink - identification of pmc1.
Hope this is helpful. Also, if you need any information about the mutants i 
have, get in touch.
madan
_______________________________________________________________________________
Subject: YPG with high CaCl2 conc.
From:    JCHO at bio1.lan.mcgill.ca at Internet
Date:    10/13/95  3:21 PM

Dear Yeast Netters,

        I am screening for calcium sensitive mutants in 300mM and 500mM
CaCl2. Calcium chloride, however, precipitates out of the media. Anybody
have any suggestions?

Thanks in advance,
John



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