I have isolated some strains which are sensitive to CaCl2 (100 and 200mM) as
suppressors of cdc1-1(ts). To prevent the precipitation of CaCl2 we use YPD
plates pH5.5 using 50mM Succinate/NaOH buffer (Calcium precipitates as its
phosphate salt and this is soluble at lower pH). This is described in Cunningham
and Fink - identification of pmc1.
Hope this is helpful. Also, if you need any information about the mutants i
have, get in touch.
Subject: YPG with high CaCl2 conc.
From: JCHO at bio1.lan.mcgill.ca at Internet
Date: 10/13/95 3:21 PM
Dear Yeast Netters,
I am screening for calcium sensitive mutants in 300mM and 500mM
CaCl2. Calcium chloride, however, precipitates out of the media. Anybody
have any suggestions?
Thanks in advance,