Does anyone have any information regarding the maximum number of plasmids
one can transform into S. cerevisiae? I have a trp-, his-, ura-, leu-
strain and would like to transform 4 constructs that carry the 4 different
markers and of course select on u-, t-, l-, h- medium. Is there anything
I should worry about? The vectors are 2 expression (CEN and 2um), 1 cDNA
library (2um), 1 reporter (CEN).
Dept. of Cell and Molecular Biology
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email: harkness at bio.bu.edu