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Solange S de Camargo scamargo at usp.br
Tue Oct 10 14:12:46 EST 1995

Hi Colleagues, 
	I have to express a specific gene in a strain without selection 
marker. So, I'd like to know if it is easy to obtain  lys - mutants and 
how I can do for get them. In addition, I'd like to know if somebody has a 
cloning vector for S. cerevisiae with the LYS2 gene as marker or the LYS2 
gene flanqued by a polilynker.  
	I'd appreciate very much if somebody could help me. 
	Best wishes, 


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