I have to express a specific gene in a strain without selection
marker. So, I'd like to know if it is easy to obtain lys - mutants and
how I can do for get them. In addition, I'd like to know if somebody has a
cloning vector for S. cerevisiae with the LYS2 gene as marker or the LYS2
gene flanqued by a polilynker.
I'd appreciate very much if somebody could help me.