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Looking for Cyclin H.....

Karl Kleine KLEINE at MIPS6.dnet.mips.biochem.mpg.de
Tue Oct 10 05:29:11 EST 1995

Several people tried already to help Charles Yang:
>On 9 Oct 1995 francis at ncbi.nlm.nih.gov wrote:
>> My colleague Karl Kleine <KLEINE at MIPS6.dnet.mips.biochem.mpg.de> replies:
>>> I am wondering where you have really searched because the CCL1 could be found
>>> in the PIR International database with accession S39383 (see entry below), 
>>> the EMBL accession is X71902 .
>> And I add:
>> Yes, I wonder myself as well, because the DNA sequence has been in the
>> GenBank (EMBL) for almost 2 years now, from the EMBL file (retrieved from 
>> the NCBI retrieve server ;-) I see:
>> ID   SCCCL1     standard; DNA; FUN; 1865 BP.
>> XX
>> AC   X71902;
>> XX
>> DT   23-DEC-1993 (Rel. 38, Created)
>> DT   07-JAN-1994 (Rel. 38, Last updated, Version 3)
>> XX
>> DE   S.cerevisiae CCL1 gene
>> This is also available from the following sources:
>> NCBI's WWW Entrez:   http://www.ncbi.nlm.nih.gov/
>> NCBI's retrieve e-mail server:  retrieve at ncbi.nlm.nih.gov 
>>        (send the word 'help' in a message to this server to 
>>         get information on how to format your queries)
>> NCBI's Network Entrez application (clients exist for Mac, 
>>                                    PC windows, Unix and others) 
>> Saccharomyces Genome Database (SGD) WWW: http://genome-www.stanford.edu/
>> Yeast Protein Database (YPD) WWW: http://quest7.proteome.com/YPDhome.html
>> MIPS/PIR WWW: http://www.mips.biochem.mpg.de/
>> Hope this helps!
>> francis
>> --
>> | B.F. Francis Ouellette  
>> | GenBank
>> |
>> | francis at ncbi.nlm.nih.gov   
>Hey guys,
>I think it would help more if you read what the guy actually wrote, not 
>what you think he wrote:
>Charles Yang <cyang at jhunix.hcf.jhu.edu> writes:
>> My problem:  I can't find the nucleotide and amino acid sequences for 
>> the Cyclin H gene (the human counterpart to CCL1) and its corresponding 
>> protein.
>I think he's trying to find the sequence of some CCL1 homolog, not CCL1.  
>Maybe he's not THAT clueless, although why post a request for a human 
>gene to yeast newsgroup...
>Mike Moser                                             Tel: 206-543-5354
>UW Department of Biochemistry                          FAX: 206-685-1792
>Box 357350                                        moser at u.washington.edu
>Seattle, WA  98195-7350                     Make peace my beast is yeast

Looking in our databases I found the Cyclin H from human is available
with accession S47592:

cyclin H - human

Species: Homo sapiens (man)

Date: 07-May-1995 #sequence_revision 21-Jul-1995 #text_change 21-Jul-

Accession: S47592

Maekelae, T.P.; Tassan, J.P.; Nigg, E.A.; Frutiger, S.; Hughes, G.J.;
    Weinberg, R.A.
    Nature 371, 254-257, 1994
    Title: A cyclin associated with the CDK-activating kinase MO15.
    Reference number: S47592
    Accession: S47592
    Status: preliminary
    Molecule type: mRNA
    Residues: 1-323 <MAE>

          20 Ala  A     10 Gln  Q     42 Leu  L     24 Ser  S
          20 Arg  R     31 Glu  E     24 Lys  K     13 Thr  T
          15 Asn  N      7 Gly  G     10 Met  M      2 Trp  W
          14 Asp  D      8 His  H     14 Phe  F     15 Tyr  Y
           8 Cys  C     14 Ile  I     15 Pro  P     17 Val  V
     Mol. wt. unmod. chain = 37,643      Number of residues = 323

              5        10        15        20        25        30
    1 M Y H N S S Q K R H W T F S S E E Q L A R L R A D A N R K F
   31 R C K A V A N G K V L P N D P V F L E P H E E M T L C K Y Y
   61 E K R L L E F C S V F K P A M P R S V V G T A C M Y F K R F
   91 Y L N N S V M E Y H P R I I M L T C A F L A C K V D E F N V
  121 S S P Q F V G N L R E S P L G Q E K A L E Q I L E Y E L L L
  151 I Q Q L N F H L I V H N P Y R P F E G F L I D L K T R Y P I
  181 L E N P E I L R K T A D D F L N R I A L T D A Y L L Y T P S
  211 Q I A L T A I L S S A S R A G I T M E S Y L S E S L M L K E
  241 N R T C L S Q L L D I M K S M R N L V K K Y E P P R S E E V
  271 A V L K Q K L E R C H S A E L A L N V I T K K R K G Y E D D
  301 D Y V S K K S K H E E E E W T D D D L V E S L

I hope this could stop the human discussion....


 Karl Kleine

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