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Binding assay to whole yeast via filtration

Eric Miska eamiska at alf2.tcd.ie
Sun Oct 8 05:21:56 EST 1995

I am interested in carrying out a tritiated-ligand binding assay on 
whole yeast cells such as that usually performed on protein/membrane 
preps by filtration via Whatman glass microfibre filters soaked in
PEI. Does anyone have experience on this, would it work?
I'd be grateful for any advice.

Cara Baker
(e-mail : crbaker at mail.tcd.ie)

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