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Unidirectional GAL1-10 promoter?

Marc Lamphier bxg00676 at niftyserve.or.jp
Sat Oct 7 12:28:43 EST 1995


In article <01HVZXXLR87691VZ85 at VAX.CS.HSCSYR.EDU>, westb at VAX.CS.HSCSYR.EDU
(Bob West) wrote:

> Dean,
> 
> We described several such constructs in our 1984 MCB paper (West, Yocum, 
> Ptashne; 4: 2467-2478), including 5' deletions of GAL1 or GAL10 and 
> internal deletions that affect one more than the other promoter (eg delta 
> 5).  These are fused to lacZ, and thus not made for overexpression 
> studies, but they or the wild-type promoters could be easily modifed for 
> such a purpose with PCR. 
> 

Hey Bob! I know that paper.....

Marc Lamphier
(now at Univeristy of Tokyo)



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