I am looking for two plasmids which I thought were relatively common but
which more local inquiries have not been able to find. I am hopeful that
this broader search will be successful.
1) pYEp51-a 2um LEU2 plasmid with GAL1-GAL10 UAS (Broach et al., Experimental
Manipulation of Gene Expression, A.P., 1983). My favorite gene will supply
2) pAAH5-a 2um LEU2 plasmid with ADH1 UAS and ADH1 mRNA terminator
downstream of the cloning site (Ammerer, Methods in Enzymology, 101:192). Again
my favorite gene will supply the ATG.
If anyone has either or both of these and would be willing to supply us with
them, I would very much appreciate hearing from you or just receiving the
plasmids or a strain containing them. Thanks very much.
Dept. of Biology
4700 Keele St.
North York, Ontario
Canada M3J 1P3
e-mail:fs300226 at sol.yorku.ca