In article <44a9j3$plq at agate.berkeley.edu>, navin <navin> wrote:
>> In order to map the binding site(s) on a receptor for a peptide
> I'm studying, I want to make a library of semi-random point-mutants, express
> this library of mutants in yeast, and then screen by FACS (using a fluorescent
> derivative of the hormone as a probe) for mutants that don't bind to the
> hormone. Our lab does not have mammalian tissue culture facilities (which is
> why we want to do our studies in yeast), but there is a departmental FACS.
> How permeable is the yeast cell wall? I realize that for
> immunofluoresence,the cell wall must be removed. But the peptides of interest
> are only 30-50 residues long (an order of magnitude smaller than antibodies).
> Would these have a problem getting through the cell wall to the plasma
> (where the receptor would be localized)?
>> Navin Pokala
>navin at uclink4.berkeley.edu
Hi. There was a thread here a couple of weeks ago on this. I think it is
generally assumed, that yeast cell wall is not a real barrier for
molecules under 1000 Da, except if a molecule-cell wall interaction
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