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Martin Kucej KUCEJ at fns.uniba.sk
Thu Oct 5 11:51:16 EST 1995

 Hello everybody,
I am trying to solve a problem of an unusual thermosensitive yeast
mutant, which lyses at 37 C. The following occurs after the shift of
this strain to the restrictive temperature:

 1. a growth ceases (cells are arrested in different stages of the
      cell  cycle)
 2. cell volume drasticly decrases (cell shrinking, cytoplasmic
      membrane separates from the cell wall)
 3. DNA content of the cell pours off to the cytoplasm (nuclear
      membrane probably disintegrates)

 Of course, this results in the death of the whole population of
cells after several hours of incubation at 37 C.
 If you have ever met such a strange mutant strain, any suggestions
or informations will be very useful for me. Thanks in advance

                  Martin Kucej

e-mail: kucej at fns.uniba.sk

Martin Kucej
Biochemistry Department
Faculty of Natural Sciences UK
Mlynska Dolina CH-1

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