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sporulation of s288c diploid

David J. Eide deide at D.UMN.EDU
Tue Oct 3 11:50:49 EST 1995


Colin,

I used to use S288c derivatives and had the same problem.  Despite repeated 
attempts, I never did get a high ratio of 4-spore tetrads so I switched to 
another background when the opportunity came along.  Perhaps someone out there 
knows the secret to success.

Dave

  In message <*-0310951548570001 at 130.216.20.102> *@sbsu1 writes:
> Dear net readers,
> I am looking for information on sporulation of a strain directly derived from
> s288c - this diploid strain seems to sporulate to give almost entirely
> three spored tetrads.  I obtained the strain by mating the FY833 and FY834
> strains, and it is WT apart from the common markers.  I am wondering if
> this incomplete sporulation occurs normally in some strain backgrounds, or
> if I'm responsible in some way.  Many thanks in advance (I will post
> replies);
> 
> Colin MacDiarmid
> Plant Science, University of Auckland.
> macdiarm at sbsu1.auckland.ac.nz
> 






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