I used to use S288c derivatives and had the same problem. Despite repeated
attempts, I never did get a high ratio of 4-spore tetrads so I switched to
another background when the opportunity came along. Perhaps someone out there
knows the secret to success.
In message <*-0310951548570001 at 188.8.131.52> *@sbsu1 writes:
> Dear net readers,
> I am looking for information on sporulation of a strain directly derived from
> s288c - this diploid strain seems to sporulate to give almost entirely
> three spored tetrads. I obtained the strain by mating the FY833 and FY834
> strains, and it is WT apart from the common markers. I am wondering if
> this incomplete sporulation occurs normally in some strain backgrounds, or
> if I'm responsible in some way. Many thanks in advance (I will post
>> Colin MacDiarmid
> Plant Science, University of Auckland.
>macdiarm at sbsu1.auckland.ac.nz>