I am interested in constructing a unidirectional GAL1-10 promoter but I
have a couple of questions. Has anyone looked at the rates of
transcription and the regulation of such a construct? I am specifically
interested in whether or not the promoter is still tightly regulated by
Gal4p and Gal80p and if there is a significant increase / decrease in the
rates of transcription from the promoter.
If anyone can provide me with any information on this I would appreciate it.
Dean H. Saxe
Dean H. Saxe "Life gives us hurdles, and we must jump,
dsaxe at bimcore.emory.edu and if you fall, you get your ass back up!"
-Millan & Kenzie "Blow"
Graduate Student, Dept. of Genetics & Molecular Biology, Emory Univ.
My home page --- <URL: http://userwww.service.emory.edu/~dsaxe>