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Plasmid integration at ura3-1 locus

Rati Verma rati at seqaxp.caltech.edu
Wed Nov 29 20:37:41 EST 1995


Gene cloned into prs306-URA3; now want to integrate into the W303 strain
backgrd which has the ura3-1 mutation. Which restriction enzyme should I
linearize the plasmid with to integrate at the mt. ura3 locus? Many thanks
in advance.



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