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Flu Tag (Tm) Affinity columns

Dr. Martin Latterich Martin_Latterich at qm.salk.edu
Tue Nov 28 18:42:07 EST 1995


Hi buddies, 

has anyone out there had any experience with Flu-tagging yeast proteins
and subsequent affinity purification over an anti-Flu antibody column?  If
yes, what is the background problem with cerevisiae expressed proteins and
the Flu epitope vs DHFR-methotrexate or His6 Ni-agarose purification
schemes?

Please reply by mail, and I will post replies in digested form.

Thank you,

Martin



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