I am doing nuclear run-ons in yeast and need a reporter construct that
has the LacZ gene with either a Gal4 or LexA DNA binding site upstream
of it. The reporters that I now have are from the Brent lab and have the
divergently transcribed Gal1/Gal10 promoters flanking the binding
site(derived from pRY131(West et al., MCB4,1984) for those familiar with
these plasmids). I need a reporter with only one promoter because of the
obvious problems presented when there is divergent transcription in the
run-on assay. Does anyone know of a reporter that fits the description?
A Ura,Trp or Leu marker is also be needed and the LexA binding site is
preferred. Thanks for any suggestions.