IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

LacZ reporters

Lynn A. Sheldon LynnA.Sheldon at dartmouth.edu
Sun Nov 26 15:10:39 EST 1995

	I am doing nuclear run-ons in yeast and need a reporter construct that 
has the LacZ gene with either a Gal4 or LexA DNA binding site upstream 
of it. The reporters that I now have are from the Brent lab and have the 
divergently transcribed Gal1/Gal10 promoters flanking the binding 
site(derived from pRY131(West et al., MCB4,1984) for those familiar with 
these plasmids). I need a reporter with only one promoter because of the 
obvious problems presented when there is divergent transcription in the 
run-on assay. Does anyone know of a reporter that fits the description? 
A Ura,Trp or Leu marker is also be needed and the LexA binding site is  
preferred. Thanks for any suggestions. 

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net