> > Hi,
> > I am currently starting expression in yeast and the manufacturer (of
> the expression system) is highly recommending baffled flasks.
> > However, we do not have those flasks, only regular Erlenmeyer. Should I
> follow blindly the manufacturer's instructions ? If not, is there
> > a place I could find some good and cheap baffled flasks (from 250 ml to
> 2000 ml) without spending away for nothing ?
>I've heard that the baffles allow for better aeration of the culture and
thus better growth. I've also headr that expression of some
difficult-to-express proteins is also better in baffled flasks. I don't
think it makes much difference in most cases, though.
>> I've only used regular Erlenmeyers and everything seems to work OK. The
> only problem seems to be a higher risk of contamination. Oh well, I
> needed practice on aseptic technique anyway.
>So, why is there an increased risk of contamination?