In article <199511221135.JAA25757 at rjo04.embratel.net.br> fleisch at embratel.net.br writes:
> Hello netters.
> Does anybody know an easy and cheap way to measure the Alcohol
> dehydrogenase activity in yeast cells?
> Thanks in advance.
>Fleisch at embratel.net.br
There is a standard yeast ADH assay in:
Methods of Enzymatic Analysis
Vol II 3rd edition 1983 - Verlag Chemie Weinheim
Section 2.2.1 pgs 139-141
Yeast ADH catalyses the oxidation of ethanol by NAD, with production of
acetaldehyde and NADH. An initial rate assay can then be used to measure the
production of NADH and from that ADH activity can be calculated. Obviously,
you'll need to disrupt the yeast cells before assaying but the assay itself
is fairly easy to carry out - tho the NAD is abit expensive.
Hope this helps (and let me know if someone comes up with a better way of
measuring yeast ADH!)
Department of Biochemical Engineering