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MET3 vs GAL1 promoter

Geoff Kornfeld geoffk at ACSUSUN.ACSU.UNSW.EDU.AU
Wed Nov 22 23:12:03 EST 1995

Mike Moser (moser at U.WASHINGTON.EDU) wrote:

:        If you want to make a strain dead, try the MET3 promoter.  It is
:much more "off" than the GAL1/GAL10 promoter.   GAL is great for making
:lots o transcript, but not so good for making no transcript.

So, is this a general offer? ;-)  Do you have a MET3 promoter vector that
you would be willing to send to people?  If so, can I be first in line?


Geoff Kornfeld					geoffk at acsusun.acsu.unsw.edu.au
Schoolof Biochemistry & Molecular Genetics,
University of New South Wales,			Tel:(61)(2) 385 2030
Sydney,						Fax:(61)(2) 385 1050
N.S.W., 2052

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