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Gal1 promoter construct of mine

killer yeast vvsvetlov at utmem1.utmem.edu
Mon Nov 20 20:22:53 EST 1995

In article <*-2111951143180001 at>, *@sbsu1.auckland.ac.nz
(*@sbsu1) wrote:

Gal1 in pYES2 appears to leak on glucose. "tight" regulation of Gal1
expression (which is not that tight) is achieved through multiple paths
working simultaneosly - down regulation of Gal4 expression, negative
regulation via Gal80p-Gal4p interactions, repression by Mig1/Ssn6/Tup1
complex etc. I would not be surprised (but too lazy to check) if
Gal1-derived promoter in pYES2 lacks Mig1p-binding sites. After all the
idea was to get overexpression vector... 


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