In article <*-2111951143180001 at 220.127.116.11>, *@sbsu1.auckland.ac.nz
Gal1 in pYES2 appears to leak on glucose. "tight" regulation of Gal1
expression (which is not that tight) is achieved through multiple paths
working simultaneosly - down regulation of Gal4 expression, negative
regulation via Gal80p-Gal4p interactions, repression by Mig1/Ssn6/Tup1
complex etc. I would not be surprised (but too lazy to check) if
Gal1-derived promoter in pYES2 lacks Mig1p-binding sites. After all the
idea was to get overexpression vector...