If you want to make a strain dead, try the MET3 promoter. It is
much more "off" than the GAL1/GAL10 promoter. GAL is great for making
lots o transcript, but not so good for making no transcript.
Mike Moser Tel: 206-543-5354
UW Department of Biochemistry FAX: 206-685-1792
Box 357350 moser at u.washington.edu
Seattle, WA 98195-7350 Make peace my beast is yeast