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Quantification of mRNA on Northern Blots

TOMASKA at fns.uniba.sk TOMASKA at fns.uniba.sk
Mon Nov 20 03:47:00 EST 1995


Dear Netters,
    My colleage is studying the effect of heat-shock on
transcription of her gene. As a standart for quantification of mRNA
on membrane she tried to use mRNA for actin. Unfortunately, the
activity of actin gene seems to be also affected by the heat
treatment. Can somebody suggest:

    1. Another acceptable standart mRNA?
    2. A possible source of a probe?

    Thank you in advance for all suggestions.

    Lubo Tomaska            tomaska at fns.uniba.sk
    Department of Genetics
    Comenius University
    Faculty of Natural Sciences
    Mlynska Dolina B-1
    842 15 Bratislava
    Slovakia




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