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Quantification of mRNA on Northern Blots

John S. J. Anderson jacobs at azstarnet.com
Mon Nov 20 09:48:14 EST 1995

In article <48pfa4$fiq at mserv1.dl.ac.uk>, TOMASKA at fns.uniba.sk wrote:


>Dear Netters,
>    My colleage is studying the effect of heat-shock on
>transcription of her gene. As a standart for quantification of mRNA
>on membrane she tried to use mRNA for actin. Unfortunately, the
>activity of actin gene seems to be also affected by the heat
>treatment. Can somebody suggest:
>    1. Another acceptable standart mRNA?

Our lab uses the 7S rRNA for quantification purposes. I'm 90% positive
that it isn't affected by heat shock.

>    2. A possible source of a probe?

Here, I don't know. We end-label an oligo we had sythnesized.

>    Thank you in advance for all suggestions.

Sure, let me know if you have any more questions.

>    Lubo Tomaska            tomaska at fns.uniba.sk
>    Department of Genetics
>    Comenius University
>    Faculty of Natural Sciences
>    Mlynska Dolina B-1
>    842 15 Bratislava
>    Slovakia
John Anderson
jacobs at azstarnet.com (<-- this is my 'home' address)
Dep't of Mol & Cell Biol
University of Arizona
Tucson USA

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