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problem with a galactose based screen

killer yeast vvsvetlov at utmem1.utmem.edu
Sun Nov 19 23:04:33 EST 1995


In article <9510131905.AA09880 at esds01.es.dupont.com>,
ballarmj at lldmpc.dnet.dupont.com (MICHELLE BALLARD) wrote:

There can be of course a copy number "problem" - something you can always
tell your boss about to keep him off your ass. This is easy to check -
reclone your cassete with insert in CEN based vector (e.g. pRS vector).
Another thing that can come into play is that even gal+ strains often grow
on galactose poorlyand therefore might perform differently depending on
transformation procedures etc. I strongly recommend for induced conditions
a mix of raffinose and galactose (3:1) as carbon source instead of
galactose alone. That should give your greater reproducibility of results.
Regards, 
Vladmeister

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