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getting rid of AD-Vectors in 2-hybrid

ehrhardt at mpimg-berlin-dahlem.mpg.de ehrhardt at mpimg-berlin-dahlem.mpg.de
Wed Nov 15 05:26:11 EST 1995


Dear netters:

Is there anyone having a faster or more convenient method for selection of
AD-vectors from 2-hybrid positive yeast clones than by selection on selective
media or using transformation of yeast DNA into Leu- deficient HB101 E.coli.
In my experience the later system suffers from low tranformation efficiency and
ultra low growth of the HB101 (up to 12days on M9-Leu minimal media).

Thanks
Tom 



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