On Tue, 7 Nov 1995, Lanes wrote:
> I have expressed a serine protease with Pichia pastoris using the pPIC9
> vector. The problem is that all proteins in the medium (BMM medium as
> described in the Pichia pastoris expression kit) precipitate. The precipitate
> is very difficult to solute and will be a big problem after upscaling the
>> What can I do to avoid this ? Please come up with some good suggestions,
> I have to purify the protein before christmas !
>> Olav Lanes
> Department of Biotechnology
> University of Tromsoe, Norway
Why don't you use a separation column before the preciptation? A
rapid separation by filtration can help you so much.