IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Pichia precipitation problem

Solange S de Camargo scamargo at usp.br
Fri Nov 10 10:26:11 EST 1995

On Tue, 7 Nov 1995, Lanes wrote:

> I have expressed a serine protease with Pichia pastoris using the pPIC9 
> vector. The problem is that all proteins in the medium (BMM medium as 
> described in the Pichia pastoris expression kit) precipitate. The precipitate
> is very difficult to solute and will be a big problem after upscaling the 
> expression.
> What can I do to avoid this ? Please come up with some good suggestions, 
> I have to purify the protein before christmas !
> Olav Lanes
> Department of Biotechnology
> University of Tromsoe, Norway
Hi Olav, 

	Why don't you use a separation column before the preciptation? A 
rapid separation by filtration can help you so much. 

	Good luck


More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net