I have devised a screening strategy to try and identify a gene of
interest from the S. cerevisiae genome, however I lack one ingredient,
that being a yeast DNA library in a LEU2 vector. I have access to one in
a URA3 vector but unfortunatly the yeast strains I am using, and need to
use, have an intact URA3 gene.
Consequently I would be greatly appreciative if anybody would be
kind enough to supply me with a yeast DNA library in a 2micron vector
with a the LEU2 selectable marker that results in a high copy plasmid
number within the cell or point me towards a suitable commercial
library (the only ones I can find are in URA3 vectors).
I hope someone can help.
Department of Clinical Biochemistry,