> From: csuzuki at nfri.affrc.go.jp (Suzuki Chise)
> I need the sequence of YEp13. From DNA database search, I got U03498
> of 10667 bp, which does not have Sac I site. However, according to
> Guide to Yeast Genetics and Molecular Biology (Method Enzymol. 194),
> YEp13 is 10786 bp and has a Sac I site. In my hands, it lookes like
> having a Sac I site.
> Does anyone know the correct sequence of YEp13?
> Thanks in advance.
if you look at this WWW page, you will find what you need (and a
little bit more ;-)
and there I found:
and the first item (<<<<<<<<<<<) is linked to the sequence. If you
don't know how to access the WWW, let me know, and I will e-mail you
I want to say a special thank you to Steve Misener
(misener at biology.queensu.ca) for putting this vector
page together! A wonderful job!
Regads to all,
| B.F. Francis Ouellette
||francis at ncbi.nlm.nih.gov
Vector IG Sequence Link : <<<<<<<
General : plasmid ds-DNA 10639 BP
Functions : (cloning)
Selection : ()
Copy Number : pMB1 25-80 copies/cell
Hosts : (E.coli C600)(E.coli K802)(E.coli)(S.cerevisiae)(Saccharomyces)
Suppliers : (ATCC)
Misc.Comments : YEp13 is mitotically stable in cir+ strains.  A YE type S.
cerevisiae/Escherichia coli shuttle vector for cloning specific genes
by transformation of yeast. Transforms leu2- yeast to Leu+ at 10E3 to
10E4 colonies per microgram of DNA. An EcoRI fragment containing the 2
micron origin was cloned into the EcoRI site of pBR322. The resulting
plasmid was partially digested with PstI, and a PstI fragment
containing yeast LEU2 cloned into the 2 micron PstI site. Restriction
digests analyzed on agarose gels give the following sizes (kb):
PstI--5.5, 4.0, 0.9; HindIII--10.7. (ATCC staff) Medium is 1227 LB plus
Parents : (pBR322)(2micron plasmid B form)
Siblings : (YEp351)(YEp352)(YEp356)(YEp366)(YEp24)
Descendents : ()