PROBLEMS WITH NOCODAZOLE

[Lisa Estey]

Hi-

	I am having problems trying to arrest G1 synchronized cerevisiae 
with nocodazole.  I'm using the drug at 20 ug/ml (dissolved in a minimal 
amount of DMSO) but I'm not really sure that it is staying in solution.
Do I need to use buffered media (I am currently using YNBD with the 
appropriate amino acid supplements)?  Is there another microtubule-specific 
agent that would be easier to use?

	Any responses posted to the newsgroup would be much appreciated!

Thanks,

Lisa Estey
Duke Univ. Medical Center/Glaxo-Wellcome, Inc.