> To: yeast at net.bio.net> From: Vermaak at micr.unp.ac.za (Kim.Vermaak)
> Subject: C. albicans cell colour
> Date: Mon, 12 Jun 1995 10:03:06 GMT
> A few weeks ago I received a culture of C. albicans to be used in
> sensitivity testing for antibiotics. I have noticed that after incubation at
> 30 degrees C for a week, the colonies on the plate start to change colour
> from creamy/white to dark olive green/black. I made a smear of some colonies
> that remained white and compared them with the darker ones. The cells of the
> darker colonies are very similar in shape and size to the cream-coloured
> ones, but their walls are much thicker and dark olive green in colour. I
> know that C. albicans has a tendency to switch colony morphologies but i
> have not heard of it changing colour in this way. Do you think it might be
> related to sporulation? Any suggestions would be greatly appreciated.
> Thanks in advance.
>> Kim Vermaak
I have never seen this happen and have cultured C. albicans for weeks
at a variety of temperatures. Even under poor growth conditions such
as on Corn Meal agar after 3-4 weeks at 25C only creamy. You either
don't have Candida albicans OR maybe your media has a different
component??? Since C. albicans only forms Chlamydospores if what you
have is sporulating it definitely isn't C. albicans.
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