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? about galactosidase assay

uno nnsw at uno.edu
Thu Jul 27 14:57:45 EST 1995

Hi, all yeasties,

I'm about to do some b-gal assay. What I'm thinking is just to 
permeabilize the cell, not to make cell extract. My question is whether I 
can freeze the samples after resuspend them in Z buffer. The protocol 
from the red book says I should place them on ice. But if I need to wait 
for a substantial amount of time before I can do the first sample, is 
there a time limit as to how long I can keep the sample on ice? If frozen 
sample is not a problem for this protocol, it will be much easier.

Thanks for your response in advance.


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