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secreted enzymes from yeasts: a request

Dan Zabetakis dan at cubsps.bio.columbia.edu
Wed Jul 19 09:58:25 EST 1995


In article <199507181727.NAA06380 at spartan.ac.brocku.ca>,
Jeffrey Atkinson <jatkin at SPARTAN.AC.BROCKU.CA> wrote:
>
>        Does anyone know of a readily available yeast (or other easily
>grown organism) that secretes an easily assayed enzyme or protein product
>into the medium?  

  In-ver-tase! In-ver-tase!

  S. cerevisiae secretes invertase (sucrose metabolism) at a high rate. You
can assay it many ways, even in a gel by running cell media.

  You might also consider alpha-factor if you are interested in the non-
beaten-path. It's a small bioactive peptide. It is assayed by it's ability
to stop cell growth on a plate.

DanZ


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